Miralys™ Neuroscience Starter Kit and sample image.
Our starter kit includes all the materials needed to implement the Miralys MALDI HiPLEX-IHC workflow in your lab, including validation slides and probe mix that allows you to conduct initial Miralys runs without using precious samples.
Kit contains:
- A UV-Photocleavage light box that has been validated for use with our workflow.
- A six-plex panel of probes; you have a choice between Neurology (mouse brain) or Immunology (human tonsil) kits.
- Three FFPE tissue slides:
- 1 slide is pre-stained with the six-plex Miralys probe mixture. This slide is provided for you to optimize your MALDI-MS imaging parameters.
- 2 slides that are unstained. These are used for you to train on the slide immunohistochemical staining protocol. They are provided from the same tissue block.
- All small laboratory items needed to run our protocol.
Your purchase of a Miralys Starter Kit comes with a high level of support, including:
- A “Pre-Staining” videoconference with an AmberGen Applications Scientist who will perform a walk-through of the protocol and provide tips and tricks to ensure the best possible experience.
- A “Post-Staining” videoconference / image review where you can share your results and an AmberGen applications scientist will offer feedback and make suggestions for fine-tuning the variables of your staining and imaging process for future runs.
- Email or telephone guidance as necessary.
Staining Protocol Practice Kit
Staining Protocol Practice Kit.
The training slides and Miralys probe mix included in the Starter Kit are available for individual purchase. This is useful as a training tool for new lab employees learning the Miralys workflow. Kits contain:
- A six-plex panel of probes; you have a choice between Neurology (mouse brain) or Immunology (human tonsil) kits
- Three FFPE tissue slides:
- 1 slide is pre-stained with the six-plex Miralys probe mixture. This slide is provided for you to optimize your MALDI-MS imaging parameters.
- 2 slides that are unstained. These are used for you to train on the slide immunohistochemical staining protocol. They are provided from the same tissue block.
Antibody Conjugation Kit
Miralys Antibody Conjugation Kit, and an example Miralys 4-plex image
The Miralys Antibody Conjugation Kit contains everything you need to create your own Miralys Probes. This includes a proprietary AmberGen photocleavable mass tag (PCMT) and all the necessary reagents and items needed to perform rapid one-step chemical conjugation of the PCMT to the antibody of your choice. More than 100 PCMTs are available for purchase to code the antibody of your choice. Each kit is designed to conjugate 100 µg of antibodies. A detailed protocol is provided, along with videoconference support.